tailieunhanh - Báo cáo khoa học: "Effect of β -mercaptoethanol or epidermal growth factor supplementation on in vitro maturation of canine oocytes collected from dogs with different stages of the estrus cycle"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Effect of β -mercaptoethanol or epidermal growth factor supplementation on in vitro maturation of canine oocytes collected from dogs with different stages of the estrus cycle | J. Vet. Sci. 2004 5 3 253-258 JOURNAL OF Veterinary Science Effect of P-mercaptoethanol or epidermal growth factor supplementation on in vitro maturation of canine oocytes collected from dogs with different stages of the estrus cycle Min Kyu Kim1 Yuda Heru Fibrianto1 Hyun Ju Oh1 Goo Jang1 Hye Jin Kim1 Kyu Seung Lee2 Sung Keun Kang1 3 Byeong Chun Lee1 3 Woo Suk Hwang1 3 4 1 Department of Theriogenology and Biotechnology College of Veterinary Medicine Seoul National University Seoul 151-742 Korea Department of Animal Science College of Agriculture and Life Sciences Chungnam National University Daejeon 305-764 Korea The Xenotransplantation Research Center Seoul National University Hospital Seoul 110-744 Korea School of Agricultural Biotechnology Seoul National University Seoul 151-742 Korea Supplementation of P-mercaptoethanol p-ME in in vitro maturation IVM medium was shown to improve embryo development and quality in several species. Epidermal growth factor EGF was also shown to improve IVM of human oocyte and embryo development after in vitro fertilization IVF . The effect of these two compounds were suggested to be mediated through the synthesis of glutathione GSH which is known to play an important role in protecting the cell or embryos from oxidative damage. Thus it is suggested that supplementation of canine IVM medium with P-ME or EGF may be of benefit due to its positive role in IVM of various mammalian oocytes and embryo development including cattle pigs rodents and humans. This study investigates the effect of ovarian estrus stage on canine oocyte quality and supplementation of medium with P-ME or EGF on IVM of canine oocytes. As results a significantly higher percentage of oocytes progressed to metaphase II Mn stage in 50 or 100 pM of P-ME supplemented oocytes collected from the follicular stage. The maturation rate to metaphase I MI stage was also significantly higher in oocytes collected from follicular stage and cultured with 25 or 100 pM compared to .