tailieunhanh - Báo cáo khoa hoc:"Expression of common fragile sites in two Ceboidea species: Saimiri boliviensis and Alouatta caraya (Primates: Platyrrhini)"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: Expression of common fragile sites in two Ceboidea species: Saimiri boliviensis and Alouatta caraya (Primates: Platyrrhini) | Genet. Sel. Evol. 32 2000 87-97 INRA EDP Sciences 87 Original article Expression of common fragile sites in two Ceboidea species Saimiri boliviensis and Alouatta caraya Primates Platyrrhini Ariela FuNDlAa Maria GoROSTlAGAb Marta MuDRYb a Department of Genetics Hematology Research Institute Mariano R. Castex National Academy of Medicine Pacheco de Melo 3081 . 1425 Buenos Aires Argentina b Department of Biology GIBE FCEyN Buenos Aires University Buenos Aires Argentina Received 7 September 1998 accepted 22 November 1999 Abstract - Fragile sites are points of preferential breakage that may be involved in chromosome rearrangements. Induction of common fragile sites c-fra and spontaneous breakage were analyzed in two New World Monkeys species Saimiri boliviensis SBO and Alouatta caraya ACA . Spontaneous chromosome aberrations were analyzed on untreated lymphocyte cultures with Brogger s formula 1977 . SBO presented a low level of spontaneous breakage while higher frequencies were detected in ACA in which bands 1q23 2q13 and 11q19 were significantly affected p . The pop-ulational distribution of c-fra was analyzed by the Chi2 test in FUdR plus caffeine treated cultures. A total of 21 c-fra was identified in SBO and 24 in ACA. Fragile sites Aiq33 Bip21 B4p14 Csq23 and Cgq22 were identified in all analyzed SBO specimens. The most frequent c-fra identified in ACA specimens were 1q23 1q31 1q33 2q22 8q14 12q31 13q22 14q15 and Xq22. Fragile sites Aiq31 Aiq33 Biq14 Bsq13 B4q21 and Xq22 identified in SBO and 1q31 1q33 2q22 4q21 6q13 13q22 and Xq22 from ACA were the most conserved sites. A low coincidence between the location of c-fra and that of heterochromatin and breakpoints involved in euchromatic rearrangements known for these genera was established. Ceboidea fragile sites chromosomal rearrangements heterochromatin evolution Résumé Sites communs de fragilite chromosomique chez deux espèces de Ceboidés Saimiri boliviensis et Alouatta caraya Primates Platyrrhini . Les .