tailieunhanh - Báo cáo khoa học: "Mucosal mast cell-derived chondroitin sulphate levels in and worm expulsion from FcRγ-knockout mice following oral challenge with Strongyloides venezuelensis"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Mucosal mast cell-derived chondroitin sulphate levels in and worm expulsion from FcRγ-knockout mice following oral challenge with Strongyloides venezuelensis | J. Vet. Sci. 2004 5 3 221-226 JOURNAL OF Veterinary Science Mucosal mast cell-derived chondroitin sulphate levels in and worm expulsion from FcRy-knockout mice following oral challenge with Strongyloides venezuelensis Denis Nnabuike Onah1 Yukifumi Nawa2 1 Department of Veterinary Parasitology Entomology University of Nigeria Nsukka Nigeria 2Department of Parasitology Miyazaki Medical College Kiyotake Miyazaki 889-1692 Japan Mucosal mast cell-derived chondroitin sulphates sulphated proteoglycans were assayed in gut washings and homogenate of FcRy-knockout KO and wild-type WT C57BL 6 mice challenged with Strongyloides venezuelensis in order to assess their possible role in secondary immunity against enteric nematodes. Groups of immune KO and WT mice were challenged by oral gavage with 300 infective larvae L3 . Establishment of infection was assessed by daily faecal analysis to determine the number of eggs per gram of faeces EPG and by adult worm recovery on days 5 and 13 post challenge. Mucosal mast cell MMC counts were done on days 5 and 13 post challenge while MMC-derived chondroitin sulphates in gut washings days 1 and 5 and homogenate day 8 were assayed by high performance liquid chromatography HPLC . Results showed that patent infection occurred in challenged KO but not WT mice despite significantly higher mastocytosis in jejunal sections of KO than WT mice p . Similarly but against prediction significantly higher concentration of MMC-derived chondroitin sulphates was observed in gut homogenate of KO than WT mice p . In contrast significantly higher concentration of chondroitin sulphates was observed in gut washings of WT than KO mice p . These results suggest that MMC in KO mice failed to release sufficient amount of sulphated proteoglycans into the gut lumen as did the WT mice which may have been part of the hostile environment that prevented the establishment in and eventual expulsion of adult S. venezuelensis from the gut of WT mice following