tailieunhanh - Báo cáo khoa học: "Characterization and localization of the unique Marek's disease virus type 2 ORF873 gene product"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Characterization and localization of the unique Marek's disease virus type 2 ORF873 gene product | J. Vet. Sci. 2004 5 3 207-213 JOURNAL OF Veterinary Science Characterization and localization of the unique Marek s disease virus type 2 ORF873 gene product Hyung-Kwan Jang Department of Infectious Diseases and Avian Diseases College of Veterinary Medicine and Bio-Safety Research Institute ChonbukNational University Jeonju 561-756 Korea Studies on Marek s disease virus MDV -unique genes are important for understanding the biological nature of the virus. Based on complete DNA sequence analyses of the MDV genomes the MDV genomes contain presumably at least five MDV-unique genes which are commonly conserved among the three MDV serotypes. A recombinant baculovirus that contains the MDV serotype 2 MDV2 -unique gene ORF873 under the polyhedrin promoter was constructed and designated rAcORF873. Polyclonal and monoclonal antibodies which recognize the recombinant MDV2 ORF873 protein in Spodoptera frugiperda clone 9 Sf9 cells infected with rAcORF873 were prepared by immunizing mice with a recombinant fusion protein expressed in Escherichia coli. Immunoblot analyses with the antibodies revealed a major protein band with a molecular mass of 108-kDa in both MDV2-infected chick embryo fibroblasts CEF and rAcORF873-infected Sf9 cells. By indirect immunofluorescence analyses using monoclonal antibody the authentic ORF873 protein was localized in the cytoplasm of MDV2-infected CEF cells. The monoclonal and polyclonal sera which were generated in the present study and reacted effectively to MDV2 ORF873 protein are considered to be useful reagents for further studying the role s of the ORF873 protein in MDV2 infection. Key words MDV MDV2 MDV unique protein MDV2 ORF873 protein Introduction Marek s disease virus MDV is an oncogenic herpesvirus which causes a highly contagious neoplastic disease in chickens. Marek s disease is characterized by the development of T cell lymphomas neurological disorders Corresponding author Phone 82-63-270-3885 Fax 82-63-270-2135 E-mail .

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