tailieunhanh - Báo cáo khoa học: "Molecular characterization of full-length genome of Japanese encephalitis virus (KV1899) isolated from pigs in Korea"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Molecular characterization of full-length genome of Japanese encephalitis virus (KV1899) isolated from pigs in Korea | J. Vet. Sci. 2004 5 3 197-205 JOURNAL OF Veterinary Science Molecular characterization of full-length genome of Japanese encephalitis virus KV1899 isolated from pigs in Korea Dong Kun Yang1 Byoung Han Kim1 Chang Hee Kweon1 Jun Hun Kwon1 Seong In Lim1 Hong Ryul Han2 National Veterinary Research and Quarantine Service Ministry of Agriculture and Forestry Anyang 430-824 Korea Department of Veterinary Internal Medicine College of Veterinary Medicine Seoul National University Seoul 151-742 Korea We have determined the complete nucleotide and deduced amino acid sequences of the Japanese encephalitis virus JEV strain KV1899 isolated from a fattening pig in Korea. In comparison with 22 fully sequenced JEV genomes currently available we found that the 10 963-nucleotide RNA genome of KV1899 has a 13-nucelotide deletion in the 3 non-translated variable region and 53 unique nucleotide sequences including 3 non-translated region NTR . Its single open reading frame has a total of 28 amino acid substitutions. Comparison of the KV1899 genomic sequence with those of the 21 fully sequenced JEV strains in published databases showed nucleotide homology ranging from Ishikawa strain to CH2195 strain . Amino acid homology with KV1899 strain ranged from K94P05 to GP78 . The KV1899 showed the highest nucleotide homology with Ishikawa strain and the highest amino acid homology with K94P05. We performed an extensive E gene based phylogenetic analysis on a selection of 41 JEV isolates available from the GenBank. Compared with Anyang strain isolated from a pig in 1969 that is current live vaccine strain for swine in Korea the homology of nucleotide sequence in envelope gene was only . The prM gene of the isolate was closely related with those of Ishikawa and K94P05 strains which were grouped into genotype I of JEV Key words Japanese encephalitis virus Complete genome sequence Phylogenetic analysis Introduction Japanese encephalitis virus JEV is a member of the family .