tailieunhanh - Báo cáo khoa học: "Detection of betanodaviruses in apparently healthy aquarium fishes and invertebrates"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Detection of betanodaviruses in apparently healthy aquarium fishes and invertebrates | J. Vet. Sci. 2006 7 4 369-374 JOURNAL OF Veterinary Science Detection of betanodaviruses in apparently healthy aquarium fishes and invertebrates Dennis Kaw Gomez1 Dong Joo Lim2 Gun Wook Baeck3 Hee Jeong Youn1 4 Nam Shik Shin1 4 Hwa Young Youn1 4 Cheol Yong Hwang1 4 Jun Hong Park2 Se Chang Park lKRF Zoonotic Disease Priority Research Institute and College of Veterinary Medicine Seoul National University Seoul 151-742 Korea department of Veterinary Medicine College of Applied Life Sciences Cheju National University Jeju 690-756 Korea 3Faculty of Marine Technology Chonnam National University Yeosu 550-749 Korea Betanodavứuses are the causative agents of vứal nervous necrosis VNN in cultured marine fish. A total of 237 apparently healthy aquarium fish marine 65 species and freshwater 12 species fishes and marine invertebrates 4 species which were stocked in a commercial aquarium in Seoul South Korea were collected from November 2005 to February 2006. The brains of the fish and other tissues of the invertebrates were examined by reverse transcriptase-polymerase chain reaction RT-PCR and nested PCR to detect betanodavirus. Positive nested PCR results were obtained from the brains of 8 marine fish species shrimp fish Aeoliscus strigatus milkfish Chanos chanos three spot damsel Dascyllus trimaculatus Japanese anchovy Engraulis japonicus pinecone fish Monocentris japonica blue ribbon eel Rhinomuraena quaesita look down fish Selene vomer yellow tang Zebrasoma flavesenes 1 marine invertebrate species spiny lobster Pamulirus versicolor and 2 freshwater fish species South American leaf fish Monocirrhus polyacanthus and red piranha Pygocentrus nattereri . The detection rate in nested PCR was 11 237 . These subclinically infected aquarium fish and invertebrates may constitute an inoculum source of betanodaviruses for cultured fishes in the Korean Peninsula. Key words aquarium fish betanodavirus invertebrate PCR detection subclinical infection vứal nervous necrosis .