tailieunhanh - Báo cáo khoa học: "Effects of DDA, CpG-ODN, and plasmid-encoded chicken IFN-γ on protective immunity by a DNA vaccine against IBDV in chickens"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Effects of DDA, CpG-ODN, and plasmid-encoded chicken IFN-γ on protective immunity by a DNA vaccine against IBDV in chickens | J. Vet. Set. 2006 7 4 361-368 JOURNAL OF Veterinary Science Effects of DDA CpG-ODN and plasmid-encoded chicken IFN-y on protective immunity by a DNA vaccine against IBDV in chickens Ha Jung Roh Haan Woo Sung Hyuk Moo Kwon School of Veterinary Medicine and Institute of Veterinary Science Kangwon National University Chuncheon 200-701 Korea This study examined the adjuvant effects of dimethyl dioctadecyl ammonium bromide DDA CpG oligodeoxynucleotides CpG-ODN and chicken interferon-y ChlFN-y on a DNA vaccine pcDNA-VP243 against the infectious bursal disease virus IBDV . A plasmid encoding chicken IFN-ã was constructed. Twice at 2-week intervals two-week-old chickens were injected intramuscularly and intraperitoneally with either a DNA vaccine alone or a DNA vaccine together with the respective adjuvants. On week 2 after the second immunization the chickens were orally challenged with the highly virulent IBDV. The groups that received the DNA vaccines plus either DDA or CpG-ODN showed significantly lower survival rates than the group that received the DNA vaccine alone. However the survival rates for the DNA vaccine alone and for the DNA vaccine plus ChlFN-y were similar. The chickens had no detectable antibodies to the IBDV before the challenge but all the surviving chickens in all groups except for the normal control group showed the induction of antibodies to the IBDV at day 10 after the challenge. As judged by the lymphocyte proliferation assays using the a WST-8 solution performed on the peripheral blood and splenic lymphocytes the stimulation indices SI of the peripheral blood lymphocytes in all groups except for the normal control group were similar immediately before the challenge. At 10 days post-challenge the SI for DNA vaccine plus either CpG-ODN or ChlFN-y was similar to that of the DNA vaccine control group. For splenic lymphocytes the SI in the DNA vaccine plus CpG-ODN and DNA vaccine plus ChlFN-y groups were higher than for the DNA vaccine control. These