tailieunhanh - Báo cáo khoa học: "Analysis of the seroprevalence of bovine paratuberculosis and the application of modified absorbed ELISA to field sample testing in Korea"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Analysis of the seroprevalence of bovine paratuberculosis and the application of modified absorbed ELISA to field sample testing in Korea | J. Vet. Sci. 2006 7 4 349-354 JOURNAL OF Veterinary Science Analysis of the seroprevalence of bovine paratuberculosis and the application of modified absorbed ELISA to field sample testing in Korea Kun Taek Park1 Jongsam Ahn3 William c. Davis4 Hye Cheong Koo2 Nam Hoon Kwon1 Woo Kyung Jung1 Jun Man Kim1 Soon Keun Hong1 Yong Ho Park1 Department of Microbiology and 2KRF Zoonotic Disease Priority Research Institute College of Veterinary Medicine Seoul National University Seoul 151-742 Korea 3Department ofBacteriology National Veterinary Research and Quarantine Service Anyang 430-824 Korea 4Department of Veterinary Microbiology and Pathology Washington State University Pullman WA 99164-7040 USA Paratuberculosis PTB is a major disease problem worldwide and causes major economic losses in the dairy industry. Although PTB has been reported in Korea no studies have been conducted to determine its prevalence and no program has been developed to control the disease. In this study the sera of beef n 1 056 and dairy cattle n 1 105 from all provinces in Korea were tested to determine the prevalence of PTB using two different ELISA an in house modified absorbed ELISA P-ELISA based on sonicated antigen from Mycobacterium avium subsp. paratuberculosis ATCC 19698 and a commercial ELISA C-ELISA . Receiver operating characteristic analysis was used to determine the cutoff point for P-ELISA. Based on C-ELISA results the area under the curve for P-ELISA was 95 CI to . Using a cutoff point of P-ELISA showed a sensitivity of and a specificity of . The kappa value and the percent agreement between the two ELISAs were and respectively. Both ELISAs showed a significant correlation between age and seropositivity p . According to C-ELISA 71 of 2 161 sera 95 CI to were test-positive. The national true prevalence of PTB was estimated to be . The findings suggest that a control program should be implemented to limit the spread of .