tailieunhanh - Báo cáo khoa học: "Gamma-ray irradiation stimulates the expression of caveolin-1 and GFAP in rat spinal cord: a study of immunoblot and immunohistochemistry"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Gamma-ray irradiation stimulates the expression of caveolin-1 and GFAP in rat spinal cord: a study of immunoblot and immunohistochemistry | J. Vet. Set. 2006 7 4 309-314 JOURNAL OF Veterinary Science Gamma-ray irradiation stimulates the expression of caveolin-1 and GFAP in rat spinal cord a study of immunoblot and immunohistochemistry Meejung Ahn1 4 Heechul Kim1 4 Jeong Tae Kim1 Jeeyoung Lee1 Jin Won Hyun2 4 Jae Woo Park3 4 Taekyun Shin14- Department of Veterinary Medicine College of Applied Life Sciences 2Department of Biochemistry College of Medicine 3 Department of Nuclear and Energy Engineering College of Engineering and4Applied Radiological Science Research Institute Cheju National University Jeju 690-756 Korea We studied the expression of caveolin-1 in the spinal cords of rats using 60Co Ỵ-ray ứradiation single dose of 8 Gray Gy in order to determine the possible involvement of caveolin-1 in the tissues of the central nervous system after irradiation. Spinal cords sampled at days 1 4 and 9 post-irradiation PI w 5 per each time point were analyzed by Western blot and immunohistochemistry. Western blot analysis showed that the expression of caveolin-1 was significantly increased at day 1 PI p and returned to the level of normal control rats on days 4 and 9 PI. Immunohistochemistry showed that caveolin-1 immunoreactivity was enhanced in some glial cells vascular endothelial cells and neurons in the spinal cords. The increased expression of glial fibrillary acidic protein GFAP a marker for an astroglial reaction was consistent with that of caveolin-1. In addition caveolin-1 was co-localized in hypertrophied GFAP-positive astrocytes. Taking all these facts into consideration we postulate that irradiation induces the increased expression of caveolin-1 in cells of the central nervous system and that its increased expression in astrocytes may contribute to hypertrophy of astrocytes in the spinal cord after irradiation. The precise role of caveolin-1 in the spinal cords should be studied further. Key words astrocyte caveolin-1 neurons radiation spinal cord Introduction Radiation has been widely used