tailieunhanh - Báo cáo khoa học: " Efficacy of VP2 protein expressed in E. coli for protection against highly virulent infectious bursal disease virus"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Efficacy of VP2 protein expressed in E. coli for protection against highly virulent infectious bursal disease virus | J. Vet. Set. 2006 7 3 241-247 JOURNAL OF Veterinary Science Efficacy of VP2 protein expressed in E coll for protection against highly virulent infectious bursal disease virus Abdul Rahman Omar1 Chong Lee Kim2 Mohd Hair Bejo2 Aini Ideris12 1 Institute of Bioscience and2Faculty of Veterinary Medicine University Putra Malaysia 43400 UPM Serdang Selangor Darul Ehsan Malaysia The ability of a heat-inactivated whole vứus from a highly vứulent infectious bursal disease virus hvIBDV and VP2 protein from hvIBDV expressed in E. coll provided protection against a hvIBDV challenge in specificpathogen-free SPF chickens. Six out of seven chickens that were injected three times with crude VP2 protein developed significant antibody titer against IBDV. However only four out of the seven chickens survived the hvIBDV challenge. Despite showing low antibody titer profiles all chickens immunized with the heat-inactivated whole virus also survived the challenged with hvIBDV. However all of these chickens had bursal atrophy and mild to moderate depletion of lymphocytes. Thus antibodies raised against IBDV VP2 protein expressed in E. coll and denatured IBDV proteins induced some degree of protection against mortality but not against bursal damage following challenge with hvIBDV. Key words antibody response E. coll heat-inactivated infectious bursal disease virus VP2 subunit vaccine Introduction Infectious bursal disease virus IBDV is one of the most important poultry viruses threatening the chicken industry worldwide. The immunosuppressive effect of this virus leads to concurrent secondary infection in the presence of other viruses or bacteria 18 . The most prominent resulting lesions are hemorrhage and necrosis of the bursa of Fabricius followed by bursal atrophy 20 . The spread of the classical and variant strains of IBDV has not been fully controlled by the introduction of attenuated live and killed variant virus vaccines 18 . Currently the major problem of IBD is controlling the .