tailieunhanh - Báo cáo khoa học: "Absence of vertical transmission of Helicobacter pylori in an experimental murine model"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Absence of vertical transmission of Helicobacter pylori in an experimental murine model | J. Vet. Set. 2006 7 3 225-228 JOURNAL OF Veterinary Science Absence of vertical transmission of Helicobacter pylori in an experimental murine model .Jin-lk Lee1 Kwonil Jung2 Okjin Kim3 Department ofLaboratory Animal Science College of Medicine Seoul National University Seoul 110-799 Korea 2New Drug Research Team Research and Development Center Daewoong Pharmaceutical Co. LTD. Yongin 449-814 Korea 3Animal Disease Research Unit College of Life Science and Natural Resources Wonkwang University Iksan 570-749 Korea Helicobacter pylori H. pylori infection is acqu ired mainly in early childhood but the precise transmission routes are unclear. This study examined the maternal H. pylori infection status in order to determine the potential of perinatal transmission. These issues were investigated using an experimental murine model the Mongolian gerbil which has been reported to be the most suitable laboratory animal model for studying H. pylori. Pregnant Mongolian gerbils infected experimentally with H. pylori were divided into two groups. The stomachs of the mother and litters were isolated and assessed for the transmission of H. pylori at the prenatal period 2 weeks after pregnancy and at the parturition day. The bacterial culture polymerase chain reaction PCR and rapid urease test were used to examine the presence of the transmitted H. pylori. There was no H. pylori observed in any of the fetuses during pregnancy and in the litters at parturition. This suggests that vertical infection during the prenatal period or delivery procedure is unlikely to be route of mother-to-child transmission of a H. pylori infection. Key words Helicobacter pylori murine model transmission vertical infection Introduction Helicobacter pylori H. pylori is a Gram-negative spiralshaped microaerophilic bacterium that infects the human gastric mucosa 8 . A chronic infection is believed to be associated with chronic active gastritis peptic ulcers and gastric malignancies such as mucosa-associated B .

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