tailieunhanh - Báo cáo khoa học: "Parentage testing of Thoroughbred horse in Korea using microsatellite DNA typing"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Parentage testing of Thoroughbred horse in Korea using microsatellite DNA typing | J. Vet. Set. 2006 7 1 63-67 JOURNAL OF Veterinary Science Parentage testing of Thoroughbred horse in Korea using microsatellite DNA typing Sun-young Lee1 Gil-jae ho2 Laboratory of Equine Genetics Korea Racing Association Gwacheon 427-711 Korea 2College of Veterinary Medicine Kyungpook National University Daegu 702-701 Korea The present study was to construct a parentage testing system for Thoroughbred TB horse. A total number of 1 285 TB horse samples including 962 foals for parentage testing 9 sires and 314 dams for individual identification were genotyped. Genomic DNA was extracted from 5 hair roots and genotyped by using 14 microsatellite markers Ain4 All 15. ASB2 ASB17 ASB23 CA425 IIMSI. HMS3 HMS6 HMS7 HTG4 HTG10 LEX3 and VHL20 . This method consisted of multiplexing PCR procedure and showed reasonable amplification of all PCR products. Genotypes were determined by genetic analyzer. The number of alleles per locus varied from 3 to 9 with a mean value of in TB horse. The expected heterozygosity was ranged from to mean and the total exclusion probability of 14 microstellite loci was . Of the 14 markers ASB2 ASB17 ASB23 HMS7 and HTG10 loci have relatively high PIC value . Of the 962 foals 960 foals were qualified by compatibility according to the Mendelism. These results suggest that the DNA typing method has high potential for parentage verification and individual identification of TB horses. Key words genotype horse microsatellite parentage verification Thoroughbred TB horse is a breed of light horses improved for a purpose of horse racing in the United Kingdom. The origin of TB horse was made by crossing eastern stallions Arabian Barb or Turk with mares of English native horse and then by the artificial selection for horse racing from early 1700 s. This breed originally a crossbred has had a closed studbook for about 200 years 20 . Korea Racing Association has played a role of the sole authority for TB horse registration in .