tailieunhanh - Báo cáo khoa học: "Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use | J. Vet. Sci. 2004 5 4 391-395 JOURNAL OF Short Communication Veterinary Science Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use Hyeong-Jun Lim1 So-Young Kim2 Wan-Kyu Lee1 1 College of Veterinary Medicine and Research Institute of Veterinary Medicine Chungbuk National University Cheongju 361-763 Korea 2Department of Food Science and Technology and Research Center for Bioresource and Health Chungbuk National University Cheongju 361-763 Korea Cholesterol-lowering effect of lactic acid bacteria LAB Streptococcus Lactobacillus and Bifidobacterium is well-known. Thus we investigated LAB isolated from human intestine on the cholesterol-lowering effect in vitro. Seven Streptococcus 11 Lactobacillus and 7 Bifidobacterium were isolated as acid pH and and bile oxgall tolerant strains. Streptococcus HJS-1 Lactobacillus HJL-37 and Bifidobacterium HJB-4 were finally selected as probiotic strains to use through the bile salt hydrolase BSH activity assay by using MRS media added taurodeoxycholic acid TDCA and the cholesterol-lowering test by using soluble cholesterol containing MRS broth. These studies suggested that the isolated LAB had an excellent hypocholesterolemic effect. Key words Lactic acid bacteria LAB probiotics cholesterol bile salt hydrolase BSH Cardiovascular disease is the most important cause of death in the westernized countries and it is strongly associated with hypercholesterolemia 17 . Decreasing serum cholesterol is therefore very important to prevent cardiovascular disease. HDL-cholesterol has been known to prevent arteriosclerosis by removing cholesterol from blood stream whereas LDL-cholesterol fastens arteriosclerosis by accumulating cholesterol in the blood vessel 16 17 . The plasma cholesterol concentration can be regulated by the biosynthesis of cholesterol from saturated fat removal of cholesterol from the circulation absorption of dietary cholesterol and excretion of cholesterol