tailieunhanh - Báo cáo khoa học: "Molecular analysis of hprt mutation in B6C3F1 mice exposed to ozone alone and combined treatment of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone and/or dibutyl phthalate for 32 and 52 weeks"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Molecular analysis of hprt mutation in B6C3F1 mice exposed to ozone alone and combined treatment of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone and/or dibutyl phthalate for 32 and 52 weeks | J. Vet. Sci. 2004 5 4 379-385 JOURNAL OF Veterinary Science Molecular analysis of hprt mutation in B6C3F1 mice exposed to ozone alone and combined treatment of 4- A7-methyl-V-nitrosamino -1- 3-pyridyl -1-butanone and or dibutyl phthalate for 32 and 52 weeks Min Young Kim1 Hyun Woo Kim1 Jin Hong Park1 Jun Sung Kim1 Hwa Jin1 Seo Hyun Moon1 Kook Jong Eu1 Hyun Sun Cho1 Gami Kang1 Yoon Shin Kim2 Young Chul Kim3 Hae Yeong Kim4 Ki-Ho Lee5 Myung Haing Cho1 1 Laboratory of Toxicology College of Veterinary Medicine and School of Agricultural Biotechnology Seoul National University Seoul 151-742 Korea 1Institute of Environmental Industrial Medicine Hanyang University Seoul 133-791 Korea 3Department of Public Health College of Natural Science Keimyung University Daegu 705-751 Korea Department of Food Science School of Biotechnology and College of Industry KyungHee University Suwon 449-701 Korea Laboratory of Molecular Cancer Biology Korea Cancer Center Hospital Seoul 139-706 Korea Potential toxicological interactions of 4-fV-methyl-V-nitrosamino -1- 3-pyridyl -1-butanone NNK and or dibuthyl phthalate DBP on ozone were investigated after 32- and 52-wk exposures using hprt mutation assay. Male and female B6C3F1 mice exposed to ozone ppm NNK mg kg DBP 5 000 ppm and two or three combinations of these toxicants 6 h per day for 32- and 52-wk showed increases in the frequencies of TGr lymphocytes compared to the control groups. Additive interactions were noted from two combination groups compared to the ozone alone in both sexes of 32- and 52-wk studies. The most common specific mutation type in the hprt genes of test materials-treated male and female mice was transversion with very few transition. The results indicate that such dominant transversion may be responsible for toxicity and combined exposure to ozone NNK and DBP induces additive genotoxicities compared to ozone alone. Key words Ozone NNK DBP hprt mutation Introduction Ozone is the major irritating oxidant gas .

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