tailieunhanh - Báo cáo khoa học: "Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis using conventional and molecular methods"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis using conventional and molecular methods | J. Vet. Sci. 2003 4 3 213-223 JOURNAL OF Veterinary Science Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis using conventional and molecular methods I. U. Khan1 A. A. Hassan2 A. Abdulmawjood2 C. Lammler3 W. Wolter4 and M. Zschock4 Department of Environmental Health Toxicology Division 3223 Eden Ave University of Cincinnati Medical Center. Cincinnati oH 45267-0056 USA 1Institutfur Tierarztliche Nahrungsmittelkunde Professur fur Milchwissenschaften Justus-Liebig-Universitat Giefien Ludwig Str 21 35390 Giefien Germany Institut fur Pharmakologie und Toxikologie Justus-Liebig-Universitat Giefien Frankfurter Str. 107 35392 Giefien Germany 3Staatliches Untersuchungsamt Hessen Marburger Str. 54 35396 Giefien Germany In the present study 130 S. uberis strains and one S. parauberis strain isolated from bovine milk samples of 58 different farms of various locations in Hesse Germany as well as two reference strains of each species were comparatively investigated for cultural biochemical serological and molecular properties. All S. uberis strains produced the enzyme P-D-glucuronidase while the strains were negative. The S. uberis and S. parauberis 16S rRNA genes were amplified by polymerase chain reaction and subsequently digested with the restriction enzymes Rsal and Avail yielding speciesspecific restriction patterns. Both species were additionally identified by amplifying species-specific parts of the genes encoding the 16S rRNA the 23S rRNA and the 16S-23S rDNA intergenic spacer region respectively. The CAMP factor gene cfu a potential virulence factor of S. uberis was amplified corresponding to a phenotypically positive CAMP-reaction using cfu-specific oligonucleotide primers. In addition the streptokinase plasminogen activator encoding genes skc pauA a second potential virulence factor could be amplified for 126 of the 130 S. uberis but not for S. parauberis. A DNA fingerprinting of S. uberis strains .