tailieunhanh - Báo cáo khoa học: "Pathological and immunohistochemical findings in broilers inoculated with a low virulent strain of infectious laryngotracheitis virus"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Pathological and immunohistochemical findings in broilers inoculated with a low virulent strain of infectious laryngotracheitis virus | J. Vet. Sei. 2003 4 2 175-180 Veterinary Science Pathological and Immunohistochemical Findings in Broilers Inoculated with a Low Virulent Strain of Infectious Laryngotracheitis Virus Necati Timurkaan Fethi Yilmaz Hakan Bulut1 Harun Ozer and Yusuf Bolat1 Department of Pathology and virology Faculty of Veterinary Medicine Firat University Elazig Turkey Received January 7 2003 Accepted July 16 2003 Abstract This study was carried out to describe clinical gross and histopathological findings in the respiratory tract in chickens infected intranasally with A96 strain of infectious laryngotracheitis virus ILTV . In addition the presence of ILTV antigens in formalin-fixed and paraffin-embedded larynx and trachea tissues was investigated with the immunoperoxidase IP method in the infected chickens. At various days of viral infection nares larynx trachea lungs and air sacs tissue samples of the infected chickens were obtained and fixed with formalin and embedded in paraffin. The cross sections were stained with hematoxylin-eosin and the larynx and trachea sections were also stained with the IP method. Mild rales and gasping were observed in only 4 of 35 chickens. The virus caused mild inflammatory changes in the respiratory tract. It was shown that clinical gross and histopathological findings were not specific for differential diagnosis of the disease. However ILTV antigens were detected by the IP method in formalin-fixed and paraffin-embedded larynx and trachea sections. These results revealed that the study use of the IP method might be useful for the diagnosis of ILTV infections with non-specific lesions. Key words infectious laryngotracheitis virus low virulent strain pathology immunoperoxidase Introduction Infectious laryngotracheitis ILT is an important respiratory disease of chickens caused by herpesvirus. It is responsible for significant mortality and loss of productivity. The disease is usually recognized as a severe necrotizing and hemorrhagic laryngotracheitis .