tailieunhanh - Báo cáo khoa học: " Reference map of soluble proteins from Salmonella enterica serovar Enteritidis by two-dimensional electrophoresis"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Reference map of soluble proteins from Salmonella enterica serovar Enteritidis by two-dimensional electrophoresis | J. Vet Sei. 2003 4 2 143-149 mmDEIB Veterinary Science Reference Map of Soluble Proteins from Salmonella enterica Serovar Enteritidis by Two-Dimensional Electrophoresis Mi-rim Park Eung-goo Lee Yong-hwan Kim Tae-sung Jung Yong-seung Shin Gee-wook Shin Hui-guen Cha and Gon-sup Kim Institute of Animal Medicine College of Veterinary Medicine Gyeongsang National University Jinju 660-701 Korea Received May 17 2003 Accepted July 23 2003 Abstract Protein identification by peptide mass fingerprinting using matrix-assisted laser desorption ionization time of fight MALDI-TOF mass spectrometry MS can analyze unambiguously identity of the spots from a 2-dimensional electrophoresis 2-DE gel. This study developed a technique for 2-DE of Salmonella enterica serovar Enteritidis S enieritidis by improving the dissolution conditions by 2-DE using a pH 4-7 immobilized pH gradient IPG strip. This report examines the protein components from the patterns of the s. enteritidis protein. The most abundant protein displayed a great number of clusters within the pH - 7 range with a molecular mass ranging from 35-80 kDa. Some of these spots were identified as metabolic related enzymes. The protein fraction was also analyzed using an immobilized pH gradient strip. Different proteins were identified on the spot according to the elongation factors. In addition this study showed that the 2-DE analysis of s. enteritidis provides useful information regarding the enteritidis proteome and this approach might provide a strategy for identifying bacterial proteins using a proteome technology. Key words salmonella enterica serovar enteritidis 2-dimensional electrophoresis 2-DE matrix-assisted laserdesorption ionization time-of-flight MALDI-TOF peptide mass fingerprinting PMF immoblilized pH gradient IPG Introduction The genus Salmonella comprises gram-negative bacilli Corresponding author Gon-sup Kim Laboratory of Veterinary BtochanisUy Gillage of Veterinaiy Medicine Gyeongsang National University .