tailieunhanh - Báo cáo khoa học: "Histochemical Detection of Glycoconjugates in the Male Reproductive System of the Horse"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Histochemical Detection of Glycoconjugates in the Male Reproductive System of the Horse | J. Vet. Sci. 2003 4 1 21-28 JOURNAL OF Veterinary Science Histochemical Detection of Glycoconjugates in the Male Reproductive System of the Horse Tae-young Ha Mee-jung Ahn Yong-duk Lee Jae-hyuk Yang Hee-seok Kim and Tae-kyun Shin Department of Veterinary Medicine Graduate School Cheju National University Jeju 690-756 Korea Received March 5 2003 Accepted April 2 2003 Abstract Lectins are glycoproteins of plant and animal origin that have the ability to bind specific carbohydrate residues of cell glycoconju gates particularly in terminal positions. In this study the binding of lectins Dolichos biflorus agglutinin DBA soybean agglutinin SBA Bandeiraea simplicifolia BS-1 isolectin B4 Triticum vulgaris WGA Arachis hypogaea PNA and Ulex europaeus UEA-I was studied in the reproductive system s of male thoroughbred horses. DBA was detected in the stereocilia of the caput and corpus epididymis and in the vas deferens. It was weakly detected in connective tissue of the corpus epididymis. Strong SBA staining was seen in epithelial cells in the testis stereocilia of the corpus and cauda epididymis and in the vas deferens. There were intense p ositive reactions for isolectin B4 in interstitial cells in all tissue and serosa of the vas deferens. PNA stainin g w as seen only in stereocilia in the caput and corpus epididymis and in the vas deferens. Strong WGA staining was seen throughout the testis except in Sertoli cells stereocilia and connective tissue. U EA-I was detected in second ary sperm atids stereocilia and epithelial cells of the cauda epididymis. These results show that degenerating cells in the testis epididym al tubules and vas deferens have differential affinities for lectins and suggest that lectins play a role in the reproductive system of the horse. The heterogeneity of the lectin staining pattern in the reproductive tubules of adult horses suggests that the carbohydrate com p osition of each ce ll type is re gion specific. Key words lectin horse testis .