tailieunhanh - Báo cáo khoa học: "Histochemical Characterization of the Lectin-binding Sites in the Equine Vomeronasal Organ"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Histochemical Characterization of the Lectin-binding Sites in the Equine Vomeronasal Organ | J. Vet. Sci. 2003 4 1 15-19 JOURNAL OF Veterinary Science Histochemical Characterization of the Lectin-binding Sites in the Equine Vomeronasal Organ Jee-young Lee Tae-young Kang Yong-duk Lee and Tae-kyun Shin Department of Veterinary Medicine College of Agriculture and Life Sciences Cheju National University Jeju 690-756 Korea Received February 28 2003 Accepted March 28 2003 Abstract The binding specificities of various lectins such as the Dolichos biflorus agglutinin DBA soybean agglutinin SBA and the Bandeiraea simplicifolia BS-1 Isolectin B4 Triticum vulgaris WGA Arachis hypogaea PNA and Ulex europaeus UEA-I lectins were studied in the vomeronasal organ of the horse. The microvilli of the vomeronasal sensory epithelium were positive for DBA SBA Isolectin B4 WGA PNA and UEA-I. The receptor cells showed intense reactivity for DBA and WGA. Lectins w ere not detected in the supporting cells or basal cells. The Jacobson s glands were positive for WGA and UEA-I but lectins were absent from the nerve bundles. From these results we postulate that several lectin-binding carbohydrates on the microvilli and neurosensory cells are associated with chemoreception in the horse. In addition the differential lectin-binding patterns in the horse suggest that the carbohydrates present in this p articular sense organ are species-specific. Key words lectin horse vomeronasal organ Introduction The vomeronasal system plays important roles in mammalian reproduction 5 . The vomeronasal organ VNO is a r ecept or organ the r ecept or cells of which pr oject their axons to the a ccessor y olfact or y bulb and participate in the perception of con -specific chemical signals pheromones 5 11 18 . The VNO has a tubular structure and its lumen is surrounded by two t ypes of epit helium the vomeronasal sensor y epithelium and the vomer onasal nonsensor y epit helium 6 15 . The vomeronasal sensory epithelium is thought to function as a signal det ect or 14 16 18 . Numerous st udies Corresponding .