tailieunhanh - Báo cáo khoa học: "Embryonic Intermediate Filaments, Nestin and Vimentin, Expression in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Embryonic Intermediate Filaments, Nestin and Vimentin, Expression in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis | J. Vet. Sci. 2003 4 1 9-13 JOURNAL OF Veterinary Science Embryonic Intermediate Filaments Nestin and Vimentin Expression in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis Tae-kyun Shin Yong-duk Lee and Ki-bum Sim1 Department of Veterinary Medicine College of Agriculture and Life Sciences Cheju National University Jeju 690-756 South Korea Department of Neurosurgery College of Medicine Cheju National University Jeju 690-756 Korea Received February 2 2003 Accepted March 18 2003 Abstract Intermediate filaments including nestin and vi-mentin are found in specific cell types in central nervous system CNS tissues particularly imm ature gli al cells and multipotent progenitor cells. In the present study the expression p atterns of nestin and vim entin in the spinal cords of rats with experimental autoimmune encephalomyelitis EAE and the response of ce lls containing filam ents against acute autoimmune inju ry were examined by immunohistochemistry. Nestin immunostaining was only w eakly detected in vascular endothelial ce lls but not in any cell types in the spinal cord in norm al and adjuvant-immunized rats. At the peak stage of EAE nestin-immunoreativity was recognized in some astrocytes in the gray m atter and w hite m atte r. Vim entin was immunopositive in some astrocytes and m acrophages in EAE lesions w hile vim entin was norm ally detected in ependym al ce lls of central canals in the rat spinal cords. We postulate that norm al anim als m ay contain multipotent progenitor cells in the spinal cord pa-renchym a as well as in the subpial lesion and epen-dym a. Multipotent progenitor ce lls may activate to transform into necessary cells including neurons astrocytes or oligodendrocytes depending on CNS needs. Appropriate control of progenitor cells in the injured CNS is an alternative choice for CNS remodeling. Key words autoimmune encephalomyelitis nestin vimentin astrocyte Introduction Experimental autoimmune encephalomyelitis EAE is an .