tailieunhanh - Báo cáo khoa học: "Mutation and overexpression of p53 as a prognostic factor in canine mammary tumors"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Mutation and overexpression of p53 as a prognostic factor in canine mammary tumors | J. Vet. Sci. 2004 5 1 63-69 JOURNAL OF Veterinary Science Mutation and overexpression ofp53 as a prognostic factor in canine mammary tumors Chung-Ho Lee Wan-Hee Kim Ji-Hey Lim Min-Soo Kang1 Dae-Yong Kim1 and Oh-Kyeong Kweon Department of Veterinary Surgery College of Veterinary Medicine Seoul National University Seoul 151-742 Korea Department of Veterinary Pathology College of Veterinary Medicine Seoul National University Seoul 151-742 Korea We concentrated ourselves to evaluate the prognostic significance of the p53 gene mutations its protein expression and MIB-1 index as a proliferative marker in canine mammary tumors. In the present study a total of 20 cases were examined among which there were 5 malignant mixed tumors 4 mammary gland adenocarcinomas 1 papillary adenocarcinoma 8 benign mixed tumors and 2 mammary gland adenomas. Positive immunostaining for p53 with PAb240 antibody was found in 2 benign 20 and 3 malignant 30 tumors. However PAb421 antibody did not give positive result at all. In Western blot analysis the p53 expression in benign and malignant tumors was detected in 4 and 3 cases respectively. p53 mutations were found in 6 cases out of the cases with detected p53 protein expression. The MIB-1 index in benign and malignant tumors were and respectively and there was no significant difference between tumor types. There was a significant correlation between p53 mutations and p53 overexpression correlation coefficient p . In Kaplan-Meier survival analysis the p53 index was associated with significantly shortened survival time p . In multivariate analysis p53 overexpression was only an independent factor for indicator of worse prognosis in canine mammary tumors p . These results demonstrated that p53 gene mutations and protein overexpression using the PAb240 anti-p53 antibody were useful predictors of increased malignant potential and poor prognosis in canine mammary tumors. Key words canine mutation overexpression