tailieunhanh - Báo cáo sinh học: " Panicovirus accumulation is governed by two membrane-associated proteins with a newly identified conserved motif that contributes to pathogenicity"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Panicovirus accumulation is governed by two membrane-associated proteins with a newly identified conserved motif that contributes to pathogenicity | Virology Journal BioMed Central Research Panicovirus accumulation is governed by two membrane-associated proteins with a newly identified conserved motif that contributes to pathogenicity Jeffrey S Batten1 2 Massimo Turina1 3 and Karen-Beth G Scholthof 1 Open Access Address Department of Plant Pathology and Microbiology Texas A M University College Station TX USA . Hawley Middle School Creedmoor NC USA and 3Istituto di Virologia Vegetale Torino Italy Email Jeffrey S Batten - battenj@ Massimo Turina - Karen-Beth G Scholthof - kbgs@ Corresponding author Published 08 March 2006 Received 15 August 2005 Accepted 08 March 2006 VirologyJournal2006 3 12 doi l 743-422X-3-12 This article is available from http content 3 1 12 2006Batten et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Panicum mosaic virus PMV has a positive-sense single-stranded RNA genome that serves as the mRNA for two 5 -proximal genes p48 and p112. The p112 open reading frame ORF has a GDD-motif a feature of virus RNA-dependent RNA polymerases. Replication assays in protoplasts showed that p48 and p112 are sufficient for replication of PMV and its satellite virus SPMV . Differential centrifugation of extracts from PMV-infected plants showed that the p48 and pll2 proteins are membrane-associated. The same fractions exhibited RNA polymerase activity in vitro on viral RNA templates suggesting that p48 and p112 represent the viral replication proteins. Moreover we identified a domain spanning amino acids 306 to 405 on the p48 and p112 PMV ORFs that is common to the Tombusviridae. Alanine scanning mutagenesis of