tailieunhanh - Báo cáo sinh học: " Involvement of PKR and RNase L in translational control and induction of apoptosis after Hepatitis C polyprotein expression from a Vaccinia virus recombinant"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Involvement of PKR and RNase L in translational control and induction of apoptosis after Hepatitis C polyprotein expression from a Vaccinia virus recombinant | BioMed Central Virology Journal Research Open Access Involvement of PKR and RNase L in translational control and induction of apoptosis after Hepatitis C polyprotein expression from a Vaccinia virus recombinant Carmen E Gómez Andrée Marie Vandermeeren María Angel García Elena Domingo-Gil and Mariano Esteban Address Department of Molecular and Cellular Biology Centro Nacional de Biotecnología CSIC Campus Universidad Autónoma 28049 Madrid Spain Email Carmen E Gómez - cegomez@ Andrée Marie Vandermeeren - avanderm@ María Angel García - magarcia@ Elena Domingo-Gil - edomingo@ Mariano Esteban - mesteban@ Corresponding author Published 12 September 2005 Received 28 July 2005 Accepted 12 September 2005 Virology Journal 2005 2 81 doi 1743-422X-2-81 This article is available from http content 2 1 81 2005 Gómez et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Hepatitis C virus HCV infection is of growing concern in public health with around 350 million chronically infected individuals worldwide. Although the IFN-a rivabirin is the only approved therapy with 10-30 clinical efficacy the protective molecular mechanism involved during the treatment is still unknown. To analyze the effect of HCV polyprotein expression on the antiviral response of the host we developed a novel vaccinia virus VV -based delivery system where structural and nonstructural except part of NS5B proteins of HCV ORF from genotype 1b are efficiently expressed and produced and timely regulated in mammalian cell lines. Results Regulated transcript production and viral polypeptide processing was demonstrated in various cell lines infected with the recombinant