tailieunhanh - Báo cáo sinh học: " Human cytomegalovirus uracil DNA glycosylase associates with ppUL44 and accelerates the accumulation of viral DNA"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Human cytomegalovirus uracil DNA glycosylase associates with ppUL44 and accelerates the accumulation of viral DNA | BioMed Central Virology Journal Research Open Access Human cytomegalovirus uracil DNA glycosylase associates with ppUL44 and accelerates the accumulation of viral DNA Mark N Prichard 1 Heather Lawlor2 Gregory M Duke2 Chengjun Mo2 Zhaoti Wang2 Melissa Dixon2 George Kemble2 and Earl R Kern1 Address Department of Pediatrics University of Alabama at Birmingham Birmingham AL USA and 2Department of Research MedImmune Vaccines Inc. Mountain View CA USA Email MarkN Prichard - mprichard@ Heather Lawlor - lawlorh@ Gregory M Duke - dukeg@ Chengjun Mo - cmo@ Zhaoti Wang - wangz@ Melissa Dixon - dixonm@ George Kemble - kembleg@ Earl R Kern - ekern@ Corresponding author Published 15 July 2005 Received 18 May 2005 Virology Journal 2005 2 55 doi 1743-422X-2-55 Accepted 15 July 2005 This article is available from http content 2 1 55 2005 Prichard et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Human cytomegalovirus ULII4 encodes a uracil-DNA glycosylase homolog that is highly conserved in all characterized herpesviruses that infect mammals. Previous studies demonstrated that the deletion of this nonessential gene delays significantly the onset of viral DNA synthesis and results in a prolonged replication cycle. The gene product pUL114 also appears to be important in late phase DNA synthesis presumably by introducing single stranded breaks. Results A series of experiments was performed to formally assign the observed phenotype to pUL114 and to characterize the function of the protein in viral replication. A cell line expressing pUL114 complemented the observed phenotype of a ULII4 deletion