tailieunhanh - Báo cáo sinh học: " A new example of viral intein in Mimivirus"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: A new example of viral intein in Mimivirus | Virology Journal BioMed Central Research A new example of viral intein in Mimivirus Hiroyuki Ogata 1 Didier Raoult2 and Jean-Michel Claverie1 Open Access Address information Génomique et Structurale UPR2589 CNRS IBSM IFR88 31 chemin Joseph Aiguier 13402 Marseille Cedex 20 France and 2Unité des Rickettsies CNRS UPRESA 6020 Faculté de Médecine 27 Boulevard Jean Moulin 13385 Marseille Cedex 05 France Email Hiroyuki Ogata - Didier Raoult - Jean-Michel Claverie - Corresponding author Published II February 2005 Received 10 January 2005 Accepted 11 February 2005 Virology Journal 2005 2 8 doi 1743-422X-2-8 This article is available from http content 2 1 8 2005 Ogata et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Inteins are protein introns that remove themselves from their host proteins through an autocatalytic protein-splicing. After their discovery inteins have been quickly identified in all domains of life but only once to date in the genome of a eukaryote-infecting virus. Results Here we report the identification and bioinformatics characterization of an intein in the DNA polymerase PolB gene of amoeba infecting Mimivirus the largest known double-stranded DNA virus the origin of which has been proposed to predate the emergence of eukaryotes. Mimivirus intein exhibits canonical sequence motifs and clearly belongs to a subclass of archaeal inteins always found in the same location of PolB genes. On the other hand the Mimivirus PolB is most similar to eukaryotic PolS sequences. Conclusions The intriguing association of an extremophilic archaeal-type intein with a mesophilic .