tailieunhanh - Báo cáo sinh học: " Murine leukemia virus (MLV) replication monitored with fluorescent proteins"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Murine leukemia virus (MLV) replication monitored with fluorescent proteins | Virology Journal BioMed Central Research Open Access Murine leukemia virus MLV replication monitored with fluorescent proteins Katja Sliva1 Otto Erlwein1 Alexandra Bittner1 and Barbara S Schnierle 1 2 Address 1Institute for Biomedical Research Georg-Speyer-Haus Paul-Ehrlich-Str. 42-44 60596 Frankfurt Main Germany and 2Paul-Ehrlich-Institute Paul-Ehrlich-Str. 51-59 63225 Langen Germany Email Katja Sliva - slika@ Otto Erlwein - erlw1@ Alexandra Bittner - alexandrabittner@ Barbara S Schnierle - schba@ Corresponding author Published 20 December 2004 Virology Journal 2004 1 14 doi 1743-422X-I-14 Received 26 November 2004 Accepted 20 December 2004 This article is available from http content 1 1 14 2004 Sliva et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Cancer gene therapy will benefit from vectors that are able to replicate in tumor tissue and cause a bystander effect. Replication-competent murine leukemia virus MLV has been described to have potential as cancer therapeutics however MLV infection does not cause a cytopathic effect in the infected cell and viral replication can only be studied by immunostaining or measurement of reverse transcriptase activity. Results We inserted the coding sequences for green fluorescent protein GFP into the prolinerich region PRR of the ecotropic envelope protein Env and were able to fluorescently label MLV. This allowed us to directly monitor viral replication and attachment to target cells by flow cytometry. We used this method to study viral replication of recombinant MLVs and split viral genomes which were generated by replacement of the MLV env gene with the red fluorescent protein RFP and separately cloning .