tailieunhanh - Báo cáo hóa học: " Effects of Proteins from Culture Medium on Surface Property of Silanes- Functionalized Magnetic "

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Effects of Proteins from Culture Medium on Surface Property of Silanes- Functionalized Magnetic | Nanoscale Res Lett 2009 4 204-209 DOI s11671-008-9226-1 NANO EXPRESS Effects of Proteins from Culture Medium on Surface Property of Silanes- Functionalized Magnetic Nanoparticles Z. P. Chen R. Z. Xu Y. Zhang N. Gu Received 22 October 2008 Accepted 24 November 2008 Published online 9 December 2008 to the authors 2008 Abstract Monodisperse magnetic nanoparticles MNPs were synthesized by thermal decomposition of iron-oleate and functionalized with silanes bearing various functional groups such as amino group NH2 short-chain poly eth-ylene glycol PEG and carboxylic group COOH . Then silanes-functionalized magnetic nanoparticles silanes-MNPs were incubated in cell culture medium plus fetal calf serum to investigate the effects of proteins from culture medium on surface property of MNPs. Zeta potential measurements showed that although surface charges of silanes-MNPs were different they exhibited negative charges at neutral pH and approximate isoelectric points after they were incubated in cell culture medium. The reason was that silanes-MNPs could easily adsorb proteins from culture medium via non-covalent binding resulting in the formation of protein-silanes-MNPs conjugates. Moreover silanes-MNPs with various functional groups had different adsorption capacity to proteins as confirmed by Coomassie blue fast staining method. The in vitro cell experiments showed that protein-silanes-MNPs had higher cellular uptake by cancer cells than silanes-MNPs. Z. P. Chen R. Z. Xu Y. Zhang N. Gu H Jiangsu Key Laboratory for Biomaterials and Devices State Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University Nanjing 210096 People s Republic of China e-mail guning@ Y. Zhang e-mail zhangyu@ Z. P. Chen School of Chemistry and Chemical Engineering Southeast University Nanjing 210096 People s Republic of China Keywords Magnetic nanoparticles Culture medium Protein Surface property Cellular uptake Introduction