tailieunhanh - Báo cáo sinh học: " Prevention of genital herpes in a guinea pig model using a glycoprotein D-specific single chain antibody as a microbicide"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Prevention of genital herpes in a guinea pig model using a glycoprotein D-specific single chain antibody as a microbicide | BioMed Central Virology Journal Research Open Access Prevention of genital herpes in a guinea pig model using a glycoprotein D-specific single chain antibody as a microbicide Jianmin Chen Sanat K Davé and Anthony Simmons Address University of Texas Medical Branch Galveston Texas USA Email Jianmin Chen - jiachen@ Sanat K Davé - skdave@ AnthonySimmons - ansimmon@ Corresponding author Published 23 November 2004 Received II November 2004 Accepted 23 November 2004 Virology Journal 2004 1 11 doi 1743-422X-1- 11 This article is available from http content I I II 2004 Chen et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Genital herpes GH is a recurrent sexually transmitted infection STI that causes significant morbidity and is also the major source of herpes simplex virus HSV in cases of neonatal herpes. Vaccination is a current goal which has had limited success so far in preventing GH and microbicides offer an attractive alternative. Treatment of primary disease cannot prevent establishment of latent infections and thus cannot prevent subsequent recurrent disease. Recently many of the molecular events leading to entry of HSV into cells have been elucidated resulting in the description of a number of herpesvirus entry mediators HVEMs that interact with HSV glycoprotein D gD on the surface of virions. Described here is a strategy for interrupting the spread of HSV based on interfering with these interactions. The hypothesis addressed in the current report was that single chain antibody variable fragments scFv that interrupt associations between gD and HVEMs would not only prevent infection in vitro but could also be used as microbicides to interfere with .