tailieunhanh - Báo cáo lâm nghiệp: "Genetic variation of Tunisian Myrtus communis L. (Myrtaceae) populations assessed by isozymes and RAPDs"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: Genetic variation of Tunisian Myrtus communis L. (Myrtaceae) populations assessed by isozymes and RAPDs. | Ann. For. Sci. 64 2007 845-853 INRA EDP Sciences 2007 DOI forest 2007061 Available online at Original article Genetic variation of Tunisian Myrtus communis L. Myrtaceae populations assessed by isozymes and RAPDs Chokri MESSA0UDa Makrem AFIFa Abdennacer B0ULILAa Mohamed Nejib REJEBb Mohamed B0USSAlDa a National Institute of Applied Science and Technology Department of Biology Laboratory of Plant Biotechnology Centre Urbain Nord BP 676 1080 Tunis Cedex Tunisia b National Institute of Research in Agricultural Engineering Water and Forests BP 10 Ariana- 2080 Tunisia Received 21 September 2006 accepted 16 July 2007 Abstract - The genetic variation of six Tunisian Myrtus communis L. Myrtaceae populations was assessed using nine isozymes coding for 17 putative loci and 79 RAPD markers amplified by five decamer random primers. The analysed populations belonged to three bioclimatic zones lower humid sub-humid and upper semi-arid . A high genetic diversity within populations was detected both by isozymes and RAPDs. The level of variation differed according to bioclimate. Populations collected from sub-humid bioclimate showed more polymorphism than those grown in the upper semi-arid zone. For all populations the genetic diversity revealed by RAPDs was more pronounced than that detected with isozymes. A high differentiation among populations related to bioclimate and geographic distance was revealed by both methods. Population s structure based on RAPD markers was more concordant with bioclimatic zones in comparison with isozymes. Differentiation between ecological groups was higher than that revealed within groups. Conservation programs should take into account the level of genetic diversity within population revealed by the two complementary classes of markers according to bioclimate. Myrtus communis RAPDs isozymes genetic diversity Tunisia Résumé - Variabilité génétique des populations tunisiennes de Myrtus communis L. Myrtaceae estimée par des .