tailieunhanh - Báo cáo hóa học: "Comparison of In vitro Nanoparticles Uptake in Various Cell Lines and In vivo Pulmonary Cellular Transport in Intratracheally Dosed Rat Model"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Comparison of In vitro Nanoparticles Uptake in Various Cell Lines and In vivo Pulmonary Cellular Transport in Intratracheally Dosed Rat Model | Nanoscale Res Lett 2008 3 321-329 DOI s11671-008-9160-2 NANO EXPRESS Comparison of In vitro Nanoparticles Uptake in Various Cell Lines and In vivo Pulmonary Cellular Transport in Intratracheally Dosed Rat Model Yurong Lai Po-Chang Chiang Jason D. Blom Na Li Kimberly Shevlin Timothy G. Brayman Yiding Hu Jon G. Selbo Liangbiao George Hu Received 7 July 2008 Accepted 18 August 2008 Published online 9 September 2008 to the authors 2008 Abstract In present study the potential drug delivery of nanoformulations was validated via the comparison of cellular uptake of nanoparticles in various cell lines and in vivo pulmonary cellular uptake in intratracheally IT dosed rat model. Nanoparticles were prepared by a bench scale wet milling device and incubated with a series of cell lines including Caco-2 RAW MDCK and MDCK transfected MDR1 cells. IT dosed rats were examined for the pulmonary cellular uptake of nanoparticles. The processes of nanoparticle preparation did not alter the crystalline state of the material. The uptake of nanoparticles was observed most extensively in RAW cells and the least in Caco-2 cells. Efflux transporter P-gp did not prevent cell from nanoparticles uptake. The cellular uptake of nanoparticles was also confirmed in bronchoalveolar lavage BAL fluid cells and in bronchiolar epithelial cells type II alveolar epithelial cells in the intratracheally administrated rats. The nanoparticles uptake in MDCK RAW cells and in vivo lung epithelial cells indicated the potential applications of nanoformulation for poorly soluble compounds. The observed limited direct uptake of nanoparticles in Caco-2 cells suggests that the improvement in oral bioavailability by particle size reduction is via increased dissolution rate rather than direct uptake. Keywords Cellular uptake Nanoparticles Intratracheally dosed rat model P-glycoprotein Abbreviations MDCK Madin-Darby canine kidney epithelial cell RAW cell The murine macrophage-like cell lines Caco-2 Human colon .