tailieunhanh - Báo cáo sinh học: " Vascular endothelial growth factor regulates melanoma cell adhesion and growth in the bone marrow"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Vascular endothelial growth factor regulates melanoma cell adhesion and growth in the bone marrow | Valcárcel et al. Journal of Translational Medicine 2011 9 142 http content 9 1 142 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Vascular endothelial growth factor regulates melanoma cell adhesion and growth in the bone marrow microenvironment via tumor cyclooxygenase-2 12 2 2 12 María Valcárcel Lorea Mendoza José-Julio Hernandez Teresa Carrascal Clarisa Salado Olatz Crende and Fernando Vidal-Vanaclocha 3 Abstract Background Human melanoma frequently colonizes bone marrow BM since its earliest stage of systemic dissemination prior to clinical metastasis occurrence. However how melanoma cell adhesion and proliferation mechanisms are regulated within bone marrow stromal cell BMSC microenvironment remain unclear. Consistent with the prometastatic role of inflammatory and angiogenic factors several studies have reported elevated levels of cyclooxygenase-2 COX-2 in melanoma although its pathogenic role in bone marrow melanoma metastasis is unknown. Methods Herein we analyzed the effect of cyclooxygenase-2 COX-2 inhibitor celecoxib in a model of generalized BM dissemination of left cardiac ventricle-injected B16 melanoma B16M cells into healthy and bacterial endotoxin lipopolysaccharide LPS -pretreated mice to induce inflammation. In addition B16M and human A375 melanoma A375M cells were exposed to conditioned media from basal and LPS-treated primary cultured murine and human BMSCs and the contribution of COX-2 to the adhesion and proliferation of melanoma cells was also studied. Results Mice given one single intravenous injection of LPS 6 hour prior to cancer cells significantly increased B16M metastasis in BM compared to untreated mice however administration of oral celecoxib reduced BM metastasis incidence and volume in healthy mice and almost completely abrogated LPS-dependent melanoma metastases. In vitro untreated and LPS-treated murine and human BMSC-conditioned medium CM increased VCAM-1-dependent BMSC adherence and .