tailieunhanh - Báo cáo sinh học: "Combination of cyclosporine and erythropoietin improves brain infarct size and neurological function in rats after ischemic stroke"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Combination of cyclosporine and erythropoietin improves brain infarct size and neurological function in rats after ischemic stroke | Yuen et al. Journal of Translational Medicine 2011 9 141 http content 9 1 141 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Combination of cyclosporine and erythropoietin improves brain infarct size and neurological function in rats after ischemic stroke Chun-Man Yuen1 Cheuk-Kwan Sun2 Yu-Chun Lin3 Li-Teh Chang4 Ying-Hsien Kao3 Chia-Hung Yen5 11 T-I I II T-I r I 7- 6 I 711 I_I Í-17- 11 u6 c 11 í n 11 - 6 I I I cl -7 I 118 _I I i I- 6 8 t Yung-Lung Chen izu-Hsien isai Sarah Chua Pei-Lin Shao Steve Leu and Hon-Kan Yip Abstract Background This study tested the superiority of combined cyclosporine A CsA -erythropoietin EPO therapy compared with either one in limiting brain infarction area BIA and preserving neurological function in rat after ischemic stroke IS . Methods Fifty adult-male SD rats were equally divided into sham control group 1 IS plus intra-peritoneal physiological saline at 24 48 h after IS group 2 IS plus CsA mg kg at 24h intra-peritoneal group 3 IS plus EPO 5 000IU kg at 24 48h subcutaneous group 4 combined CsA and EPO same route and dosage as groups 3 and 4 treatment group 5 after occlusion of distal left internal carotid artery. Results BIA on day 21 after acute IS was higher in group 2 than in other groups and lowest in group 5 all p . The sensorimotor functional test showed higher frequency of left turning in group 2 than in other groups and lowest in group 5 all p . mRNA and protein expressions of apoptotic markers and number of apoptotic nuclei on TUNEL were higher in group 2 than in other groups and lowest in group 1 and 5 whereas the anti-apoptotic markers exhibited an opposite trend all p . The expressions of inflammatory and oxidized protein were higher in group 2 than in other groups and lowest in group 1 and 5 whereas anti-inflammatory markers showed reversed changes in group 1 and other groups all p . The number of aquaporin-4 and glial fibrillary acid protein .