tailieunhanh - Báo cáo sinh học: "The immunological potency and therapeutic potential of a prototype dual vaccine against influenza and Alzheimer’s disease"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: The immunological potency and therapeutic potential of a prototype dual vaccine against influenza and Alzheimer’s disease | Davtyan et al. Journal of Translational Medicine 2011 9 127 http content 9 1 127 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access The immunological potency and therapeutic potential of a prototype dual vaccine against influenza and Alzheimer s disease 1 3 3 2 2 Hayk Davtyan 1 Anahit Ghochikyan Richard Cadagan Dmitriy Zamarin Irina Petrushina Nina Movsesyan Luis Martinez-Sobrido4 Randy A Albrecht3 5 Adolfo García-Sastre3 5 6 and Michael G Agadjanyan1 2 Abstract Background Numerous pre-clinical studies and clinical trials demonstrated that induction of antibodies to the p-amyloid peptide of 42 residues Ap42 elicits therapeutic effects in Alzheimer s disease AD . However an active vaccination strategy based on full length Ap42 is currently hampered by elicitation of T cell pathological autoreactivity. We attempt to improve vaccine efficacy by creating a novel chimeric flu vaccine expressing the small immunodominant B cell epitope of Ap42. We hypothesized that in elderly people with pre-existing memory Th cells specific to influenza this dual vaccine will simultaneously boost anti-influenza immunity and induce production of therapeutically active anti-Ap antibodies. Methods Plasmid-based reverse genetics system was used for the rescue of recombinant influenza virus containing immunodominant B cell epitopes of Ap42 Ap1-7 10 . Results Two chimeric flu viruses expressing either 7 or 10 aa of Ap42 flu-Ap1-7 or flu-Ap1-10 were generated and tested in mice as conventional inactivated vaccines. We demonstrated that this dual vaccine induced therapeutically potent anti-Ap antibodies and anti-influenza antibodies in mice. Conclusion We suggest that this strategy might be beneficial for treatment of AD patients as well as for prevention of development of AD pathology in pre-symptomatic individuals while concurrently boosting immunity against influenza. Introduction Alzheimer s disease AD is the most common form of dementia in the elderly