tailieunhanh - Báo cáo sinh học: "Glycyrrhizin as antiviral agent against Hepatitis C Virus"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Glycyrrhizin as antiviral agent against Hepatitis C Virus | Ashfaq et al. Journal of Translational Medicine 2011 9 112 http content 9 1 112 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Glycyrrhizin as antiviral agent against Hepatitis C Virus Usman A Ashfaq1 Muhammad S Masoud1 Zafar Nawaz2 and Sheikh Riazuddin3 Abstract Background Hepatitis C virus is a major cause of chronic liver diseases which can lead to permanent liver damage hepatocellular carcinoma and death. The presently available treatment with interferon plus ribavirin has limited benefits due to adverse side effects such as anemia depression fatigue and flu-like symptoms. Herbal plants have been used for centuries against different diseases including viral diseases and have become a major source of new compounds to treat bacterial and viral diseases. Material The present study was design to study the antiviral effect of Glycyrrhizin GL against HCV. For this purpose HCV infected liver cells were treated with GL at non toxic doses and HCV titer was measured by Quantitative real time RT-PCR. Results and Discussion Our results demonstrated that GL inhibit HCV titer in a dose dependent manner and resulted in 50 reduction of HCV at a concentration of 14 2 qg. Comparative studies were made with interferon alpha to investigate synergistic effects if any between antiviral compound and interferon alpha 2a. Our data showed that GL exhibited synergistic effect when combined with interferon. Moreover these results were verified by transiently transfecting the liver cells with HCV 3a core plasmid. The results proved that GL dose dependently inhibit the expression of HCV 3a core gene both at mRNA and protein levels while the GAPDH remained constant. Conclusion Our results suggest that GL inhibit HCV full length viral particles and HCV core gene expression or function in a dose dependent manner and had synergistic effect with interferon. In future GL along with interferon will be better option to treat HCV infection. Background Hepatitis