tailieunhanh - Báo cáo sinh học: " Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneratio"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneratio | Liu et al. Journal of Translational Medicine 2011 9 111 http content 9 1 111 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneration 11 2 111 1 Baoying Liu Lai Wei Catherine Meyerle Jingsheng Tuo H Nida Sen Zhiyu Li Sagarika Chakrabarty 2 1 3 2 2 1 Elvira Agron Chi-Chao Chan Michael L Klein Emily Chew Frederick Ferris and Robert B Nussenblatt Abstract Background Age related macular degeneration AMD is the leading cause of irreversible blindness in elderly populations worldwide. Inflammation among many factors has been suggested to play an important role in AMD pathogenesis. Recent studies have demonstrated a strong genetic association between AMD and complement factor H CFH the down-regulatory factor of complement activation. Elevated levels of complement activating molecules including complement component 5a C5a have been found in the serum of AMD patients. Our aim is to study whether C5a can impact human T cells and its implication in AMD. Methods Human peripheral blood mononuclear cells PBMCs were isolated from the blood of exudative form of AMD patients using a Ficoll gradient centrifugation protocol. Intracellular staining and enzyme-linked immunosorbent assays were used to measure protein expression. Apoptotic cells were detected by staining of cells with the annexin-V and TUNEL technology and analyzed by a FACS Caliber flow cytometer. SNP genotyping was analyzed by TaqMan genotyping assay using the Real-time PCR system 7500. Results We show that C5a promotes interleukin IL -22 and IL-17 expression by human CD4 T cells. This effect is dependent on B7 IL-1Ị3 and IL-6 expression from monocytes. We have also found that C5a could protect human CD4 cells from undergoing apoptosis. Importantly consistent with a role of C5a in promoting IL-22 and IL-17 expression significant elevation in IL-22 and .