tailieunhanh - Báo cáo khoa học: Transcript profiling reveals diverse roles of auxin-responsive genes during reproductive development and abiotic stress in rice

Auxin influences growth and development in plants by altering gene expression. Many auxin-responsive genes have been characterized in Ara-bidopsisin detail, but not in crop plants. Earlier, we reported the identifi-cation and characterization of the members of the GH3, Aux⁄IAA andSAURgene families in rice. | Transcript profiling reveals diverse roles of auxin-responsive genes during reproductive development and abiotic stress in rice Mukesh Jain1 and Jitendra P. Khurana2 1 National institute of Plant Genome Research NIPGR Aruna Asaf Ali Marg New Delhi India 2 Interdisciplinary Centre for Plant Genomics and Department of Plant Molecular Biology University of Delhi South Campus New Delhi India Keywords abiotic stress auxin microarray analysis reproductive development rice Oryza sativa Correspondence M. Jain National institute of Plant Genome Research NIPGR Aruna Asaf Ali Marg New Delhi-110067 India Fax 91 11 26741658 Tel 91 11 26735182 E-mail mjain@ mjainanid@ Received 1 January 2009 revised 2 March 2009 accepted 31 March 2009 doi Auxin influences growth and development in plants by altering gene expression. Many auxin-responsive genes have been characterized in Ara-bidopsis in detail but not in crop plants. Earlier we reported the identification and characterization of the members of the GH3 Aux IAA and SAUR gene families in rice. In this study whole genome microarray analysis of auxin-responsive genes in rice was performed with the aim of gaining some insight into the mechanism of auxin action. A comparison of expression profiles of untreated and auxin-treated rice seedlings identified 315 probe sets representing 298 225 upregulated and 73 downregulated unique genes as auxin-responsive. Functional categorization revealed that genes involved in various biological processes including metabolism transcription signal transduction and transport are regulated by auxin. The expression profiles of auxin-responsive genes identified in this study and those of the members of the GH3 Aux IAA SAUR and ARF gene families were analyzed during various stages of vegetative and reproductive panicle and seed development by employing microarray analysis. Many of these genes are indeed expressed in a tissue-specific or developmental .