tailieunhanh - Báo cáo khoa học: " Sequence Analysis of Canine LINE-1 Elements and p53 Gene in Canine Transmissible Venereal Tumor"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Sequence Analysis of Canine LINE-1 Elements and p53 Gene in Canine Transmissible Venereal Tumor | J. Vet. Sci. 2002 3 4 285-292 JOURNAL OF Veterinary Science Sequence Analysis of Canine LINE-1 Elements and p53 Gene in Canine Transmissible Venereal Tumor Young-Ki Choi and Chul-Joong Kim Department of Clinical and Population Sciences College of Veterinary Medicine University of Minnesota 1988 Fitch Avenue MN 55108 Laboratory of Infectious Diseases College of Veterinary Medicine Chungnam National University Daejeon 305-764 Korea Received June 7 2002 Accepted November 15 2002 Abstract LINEs long interspersed nuclear elements or long interspersed repeated DNA elements contains two open reading frames ORFs ORF1 and ORF2. We analysed the ORF2 located in the 5 re gion to the first exon of oncogene c-myc in canine transmissible venereal tumor TVT cell. We also showed the transcription activation was induced by this TVT-LINE sequence using CAT assay. To identify the mutation of tum or suppressor gene sequence analysis of p53 from TVT cell w as performed. We identified the point mutation of 964 nucleotide T C resulting in the change of amino acid Phe Ser of p53 tumor suppressor protein. Introduction Mammalian genomic DNA contains several families of frequently repeated sequences and some have been shown to be mobile elements such as SINE and LINE 9 21 . They are known to be mostly flanked by dir ect repeat s of 7-20 base pair s bp that are believed to r esult in the duplication of target site. LINEs long interspersed nuclear element s or long int er sper sed repeated DNA elements are consider ed to be a nonviral retroposon found in all mammals. The LINE-1 elements which are mostly truncated at the 5 end existed on the genomic DNA with 10 000 to 50 000 copies 5 and they encode two open reading frames ORFs ORF1 and ORF2. From the results of sequence analysis and purified component s the ORF2 of the LINE-1 encodes such biological functions as r ever se transcriptase endonuclea se and possibly RNase H 8 15 17 . In contrast the ORF1 has no activity showing a known .