tailieunhanh - Báo cáo khoa học: "Immunohistochemical Localization of Bcl-2 in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Immunohistochemical Localization of Bcl-2 in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis | J. Vet. Sci. 2002 3 4 279-283 JOURNAL OF Veterinary Science Immunohistochemical Localization of Bcl-2 in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis Chang-Jong Moon Yong-Duk Lee and Tae-Kyun Shin Department of Veterinary Medicine Cheju National University Jeju 690-756 Korea Received May 3 2002 Accepted November 14 2002 Abstract We examined the localization of the anti-apoptotic molecule Bcl-2 in the spinal cords of Lewis rats with experimental autoimmune encephalomyelitis EAE . Western blot analysis showed that Bcl-2 was constitutively expressed in norm al spinal cords and w eakly increased in response to com ple te Freund s adjuvant CFA immuniz ation. In EAE with infiltration of inflamm atory cells into spinal cords Bcl-2 declined during the peak stage and further decreased during the recovery stage. Immunohistochemically some neurons and glial cells constitutively expressed Bcl-2 in norm al rat spinal cords. In the spinal cords of rats with EAE Bcl-2 was also immunoreacted in some perivascular inflamm atory ce lls w hile some brain cells such as neurons and GFAP astrocytes show ed less Bcl-2 immunoreaction. These findings suggest that in EAE Bcl-2 expression in the CNS host cells decreases with CNS inflammation possibly progressing to cell death in some cases w hile the survival of host cells including neurons astrocytes and some inflamm atory cells is associated with activation of the anti-apoptotic molecule Bcl-2. Taking all into considerations its is postulated that Bcl-2 either beneficially or detrim entally functions in some host cells depending on the activation stage of each cell type. Key words apoptosis autoimmune encephalomyelitis Bcl-2 Introduction Experimental autoimmune encephalomyelitis EAE is an autoimmune disease of the central nervous system CNS Corresponding author Tae-Kyun Shin Department of Veterinary Medicine Cheju National University Jeju 690-756 Korea Tel 82-64-754-3363 Fax 82-64-756-3354 E-mail shint@