tailieunhanh - Báo cáo khoa học: "Genetic Polymorphism of the Serum Proteins of Horses in Jeju"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Genetic Polymorphism of the Serum Proteins of Horses in Jeju | J. Vet. Sci. 2002 3 4 255-263 JOURNAL OF Veterinary Science Genetic Polymorphism of the Serum Proteins of Horses in Jeju Jin-Ah Shin1 Young-Hoon Yang2 Hee-Seok Kim1 Young-Min Yun1and Kyoung-Kap Lee1 1Department of Veterinary Medicine Agriculture Life Sciences Cheju National University Jeju Korea 2Department of Animal Biotechnology Agriculture Life Sciences Cheju National University Jeju Korea Received June 27 2002 Accepted November 8 2002 Abstract The study was carried out to investigate the genetic polymorphism of the serum proteins of horses in Cheju. They were assigned to three groups 45 Cheju native horses CNH 60 Cheju racing horses CRH and 60 Thoroughbreds TB . We analyzed the phenotypes and gene frequencies of serum proteins which were album in Alb vitamin-D binding protein GC esterase ES A1B glycoprotein A1B and transferrin TF loci using horizontal polyacrylamide gel electrophoresis HPAGE . All of the loci except A1B in TB showed p olym or-phisms and different allelic and phenotypic frequencies in all three groups. ESS and TFF1 were not observed in CNH. Allelic frequencies of AlbB ESI TFDand TFF1 were high in TB. All of the loci except ES locus in CRH appeared to be in a state of Hardy-Weinberg equilibrium from goodness-of-fit test in all three groups Heterozygosity estimates at Alb ES and TF loci were high but GC and A1B loci were low in all three groups. Average heterozygosities in CNH CRH and TB were and respectively. Results show ed differences in the frequencies of alleles and phenotypes of several serum protein loci betw een CNH and CRH suggested that CRH might be crossed with other breeds of horses in some degree. Key words serum protein polymorphism phenotype frequency heterozygosity HPAGE Cheju native horse Introduction The Cheju native horses CNH are representative of the native horses in Korea and have a particular hereditariness in pr ocess of adaptation to the clim at e of Cheju. In recent years it has been assumed that some