tailieunhanh - Báo cáo khoa học: "Immunohistochemistry of Voltage-Gated Calcium Channel α1B Subunit in Mouse Cerebellum"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Immunohistochemistry of Voltage-Gated Calcium Channel α1B Subunit in Mouse Cerebellum | J. Vet. Sci. 2002 3 3 175-178 JOURNAL OF Veterinary Science Immunohistochemistry of Voltage-Gated Calcium Channel 115 Subunit in Mouse Cerebellum Seung-Hyuk Chung Kyung-Youl Lee Ki-Hyung Kim Chul-Tae Kim Nam-Seob Lee Kazuhiko Sawada1 1 2 .1 3 Hiromi Haga Byung-Chul Lee Yoshihiro Fukui Man-Hee Rhee and Young-Gil Jeong Department of Anatomy College of Medicine Konyang University Nonsan Chungnam 320-711 South Korea 1Department of Anatomy University of Tokushima School of Medicine Tokushima 770-8503 Japan 2Cancer Biology and Neuroscience Research Groups The University of Calgary Calgary Alberta Canada T2N 4N1 3Department of Cell Biology Physiology Washington University School of Medicine St. Louis MO 63110 USA Received May 9 2002 Accepted August 21 2002 Abstract Secretion of neurotransmitters is initiated by voltage-gated calcium influx through presynaptic voltagegated N-type calcium channels. However little is known about their cellular distribution in the mouse cerebellum. In the cerebellum a IB immunoreactivity is found mainly on the cell bodies of all Purkinje cells. In addition the immunoreactivity was detected on a subset of Purkinje cell dendrites clustered to form a parasagittal array of bands. In the anterior lobe vermis immunoreactive Purkinje cell dendrites form narrow stripes separated by broad bands of unstained dendrites. Moving caudally through the vermis these stripes become thicker as a larger fraction of the Purkinje cell dendrites become immunoreactive. This localization study of the Ơ1B pore-forming subunits in mouse cerebellum may guide future investigations of the role of calcium channels in neurological pathways. Key words N-type calcium channels Purkinje cell Cerebellum Introduction Calcium ions play a major role in many different physiological functions including synaptic transmitter release and membrane excitability 2 neurite outgrowth This study was supported by grants from the Basic Research Program of the Korea Science Engineering .