tailieunhanh - Báo cáo khoa học: "Prior Ischemic Treatment Renders Kidney Resistant to Subsequent Ischemia"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Prior Ischemic Treatment Renders Kidney Resistant to Subsequent Ischemia | J. Vet. Sci. 2002 3 2 115-121 JOURNAL OF V eterinary Science Prior Ischemic Treatment Renders Kidney Resistant to Subsequent Ischemia Kwon-Moo Park and Ho-Jae Han Department of Veterinary Physiology College of Veterinary Medicine Biotechnology Research Institute Chonnam National University Kwangju 500-757 Korea Received Mar. 6 2002 Accepted May 29 2002 ABSTRACT Prior ischemia leads to resistance against subsequent ischemic insults. The mechanisms that underlie this adaptive response rem ain unidentified. Thus we studied whether the reduced susceptibility of mice previously subjected to the ischemia to ischemia reperfusion inju ry is related with alte re d inflammatory responses. Thirty minutes of bilateral kidney ischemia results in significantly increased plasm a creatinine and blood urea nitrogen levels in BALB c male mice. There is severe disruption of actin cytoskeleton of proximal tubular cells in the outer strip e of the outer medulla 24 hours post-ischemia. When mice are subjected to 30 minutes of bilateral ischemia 8 days later there is no increase in plasma creatinine and blood urea nitrogen levels and the post-ischemic disruption of actin cytoskeleton of proximal tubular ce lls is much less. Inflammatory responses have highly implicated w ith ischemia reperfusion inju ry. Ischemia results in the increased tissue myeloperoxidase MP O activity that is a marker of leukocyte infiltration. There is how ever no the post-ischemic increase of MPO activity in kidneys previously subjected to ischemia. Post-ischemic expression of tissue intercellular adhesion molecule-1 ICAM-1 is greater in the kidney previously sham-operated than in the kidneys previously subjected to ischemia. In conclusion prior ischemia protects kidney function and m orphology against subsequent ischemia 8 days later. The resistance is associated with the reduced post-ischemic leukocyte infiltration due to the reduced post-ischemic ICAM-1 expression. Key words Ischemia Inflammation Intercellular