tailieunhanh - Báo cáo khoa học: "Isolation of Subgroup J Avian Leukosis Virus in Korea"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Isolation of Subgroup J Avian Leukosis Virus in Korea | J. Vet. Sci. 2002 3 2 71-74 JOURNAL OF Veterinary Science Isolation of Subgroup J Avian Leukosis Virus in Korea Haan-Woo Sung Jae-Hong Kim Sanjay Reddy1 and Aly Fadly1 National Veterinary Research and Quarantine Service Ministry of Agriculture Forestry 480 Anyang 6-Dong Anyang 430-016 Korea . Department of Agriculture Agricultural Research Service Avian Disease and Oncology Laboratory 3606 East Mount Hope Road East Lansing MI 48823 Received Feb. 1 2002 Accepted Apr. 25 2002 ABSTRACT Two subgroup J avian leukosis viurses ALVs were isolated from broiler breeder flocks in which myeloid leukosis had occurred. The isolates could be classified as subgroup J ALV by the positive reaction in p olym erase ch ain reaction P CR with primers specific for subgroup J ALV. Two isolate s replicated in chicken em bryo fibroblast CEF cells from the alv6 chicken line in w h i c h c e l ls a re re s i s t a n t t o s u b g ro u p A a n d E ALVs . In in vitro serum neutralization tests with other subgroup ALVs including ADOL-Hc1 the prototype of subgroup J ALVs isolated in the United States of America two isolates were partially neutralized by antibody to ADOL-Hc1 indicating that Kore an isolates and ADOL-Hc1 may be antigenically related but not identical. When the PCR was done with a primer pair designed to amplify genes of E elem ent and long terminal repeat of p roviral DNA the PCR product size of one isolate KOAL-P ET was sm aller than that of ADOL-Hc1 suggesting that some sequences in these regions are deleted. Key words subgroup J avian leukosis virus myeloid leukosis virus isolation Introduction Avian leu kosis is a n eopla st ic disease cau sed by avian leukosis viruses ALVs . ALVs from chickens have been divided into six subgroups A B C D E and J on the basis of th eir host range vir al envelope interference and cross-neutralization pattern s 10 . Subgroups A B C and D are exogen ou s vir u ses ca pa ble of inducing t umor s and subgroup E vir uses are endogenous vir uses .