tailieunhanh - Báo cáo khoa học: " Effect of IP3 and ryanodine treatments on the development of bovine parthenogenetic and reconstructed embryos"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Effect of IP3 and ryanodine treatments on the development of bovine parthenogenetic and reconstructed embryos | J. Vet. Sci. 2001 2 2 131-137 OURNAL OF Veterinary Science Effect of IP3 and ryanodine treatments on the development of bovine parthenogenetic and reconstructed embryos Gook-jun Ahn Byeong-chun Lee and Woo-suk Hwang Department of Theriogenology College of Veterinary Medicine Seoul National University Seoul 151-742 Korea For parthenogenetic activation as a model system of nuclear transfer microinjection and electroporation as activation treatments in bovine metaphase II oocytes were administered to each of three groups as follows control group treatments with Ca2 Mg2 -free PBS 100 pM EgTA IP3 group control 25 pM IP3 and IP3 ryanodine group control 25 pM IP3 10 mM ryanodine . In experiments using microinjection no significant differences were observed between any of the developmental stages of the electroporation experiment. For electroporation cleavage rates were significantly higher in the IP3 ryanodine group than in the IP3 or control group vs or respectively . In the subsequent stages of embryonic development such as morula and blastocyst formation the IP3 and ryanodine group exhibited significantly higher rates of morula fomation than the IP3 or control groups vs or respectively . Similarly the rate of blastocyst formation in the IP3 ryanodine group was significantly higher than the control group vs but did not differ significantly from the IP3 group vs . In nuclear transfer activation was performed at 30 hpm by microinjection and elecroporation with 25 pM IP3 10 mM ryanodine followed by 6-DMAP treatment. No significant differences were observed at any stage of embryonic development and none of the embryos activated by electroporation reached either the morula or blastocyst stage. However and of embryos activated by microinjection sucessfully developed to the morula and blastocyst stages respectively. In conclusion activation treatments using IP3 and ryanodine are able to support the development of bovine .