tailieunhanh - Báo cáo khoa học: "Experimental autoimmune encephalomyelitis in cynomolgus monkeys"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Experimental autoimmune encephalomyelitis in cynomolgus monkeys | J. Vet. Sci. 2000 1 2 127-131 OURNAL OF Veterinary Science Experimental autoimmune encephalomyelitis in cynomolgus monkeys Ki-Hoan Nam Division of Rheumatology Department of Internal Medicine University of Iowa Iowa City IA 52242. . Experimental autoimmune encephalomyelitis was induced in macaques. T cell clones infiltrated into the brain lesion area were compared with those in blood. Intradermal immunization of macaques with brain white matter derived from healthy macaque in combination with pertussis toxin induced neurological symptoms in two macaques. One died on day 25 after immunization whereas the other survived. Gross examination of the brain from the dead macaque showed clear hemorrhagic lesions in the white matter. Hematological analysis showed that drastic T cell response was induced in macaques immunized with white matter but not in control macaques. Flow cytometric analysis of blood cells from the affected macaques demonstrated an increase of CD4 and CD8 T cell populations expressing the CD69 early activation marker. Single strand conformation polymorphism SSCP analysis of T cell receptor beta chain showed T cell clones infiltrated into the brain lesion which were different from those found in the peripheral blood of the same monkey. The present paper shows that SSCP analysis of TCR is useful in studing clonality of T cells infiltrating into the brain tissue of macaque with EAE. Key word EAE in cynomolgus monkeys Introduction Experimental autoimmune encephalomyelitis EAE an animal model of multiple sclerosis MS in humans a spontaneous inflammatory demyelinating disease of young adults results from immunization with central nervous system CNS myelin proteins or peptides under Th1 conditions or by the adoptive transfer of CNS Ag-specific CD4 Th1 cells 1 21 22 . The process involveds the death of cellular constituents of the CNS including oligodendroglia and neurons as well as damage to myelin. The majority of EAE models have been developed in .