tailieunhanh - Báo cáo khoa học: "Effect of ethylene glycol monoethyl ether on the spermatogenesis in pubertal and adult rats"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Effect of ethylene glycol monoethyl ether on the spermatogenesis in pubertal and adult rats | J. Vet. Sci. 2001 2 1 47-51 JOURNAL OF Veterinary Science Effect of ethylene glycol monoethyl ether on the spermatogenesis in pubertal and adult rats Chang Yong Yoon Choong Man Hong1 Ji Young Song1 Yong-Yeon Cho Kwang-Sik Choi Beom Jun Lee2 and Cheol Kyu Kim1 Kwangju Regional Food Drug Administration Kwangju 500-480 Korea. Department of Pathology National Institute of Toxicological Research Korea Food Drug Administration Seoul 122-704 Korea. Safety Research Center Korea Testing and Research Institute for Chemical Industry Seoul 150-038 Korea The effects of ethylene glycol monoethyl ether EGEE on testicular cell populations in pubertal 5 weeks old and adult 9 weeks old male rats were investigated by a flow cytometric method. A total of 50 rats in number 25 pubertal and 25 adult rats was divided into 5 experimental groups including 0 control 50 100 200 and 400 mg EGEE kg of body weight. The animals were administered by gavage for 4 weeks. In adult rats the treatment of EGEE at the dose of 400 mg kg of body weight decreased significantly the populations of haploid while it increased those of diploid and tetraploid cells. In pubertal rats the treatment of EGEE at the dose of 400 mg kg of body weight caused only minimal changes in the relative percent of testicular cell types. These results suggest that the effects of EGEE on testicular function in pubertal rats appear to be less pronounced than in adult rats. Key words flow cytometry ethylene glycol monoethyl ether testis epididymis DNA contents Introduction Testicular damage by a toxicant is evaluated by analyzing parameters such as fertility pregnancy outcome testicular cell morphology and sperm motility 7 9 12 . Traditional approaches involve histopathologic examination of testicular tissue which includes the description of several cell types the determination of spermatogenic stages and the detection of morphologic and cell-kinetic abnormalies in the sper-matogenic process 13 . However these methods are subjective