tailieunhanh - Báo cáo khoa học: "Using pig biliary system, in vivo propagation of Enterocytozoon bieneusi, an AIDS-related zoonotic pathogen"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Using pig biliary system, in vivo propagation of Enterocytozoon bieneusi, an AIDS-related zoonotic pathogen | J. Vet. Sci. 2000 1 2 105-111 OURNAL OF Veterinary Science Using pig biliary system in vivo propagation of Enterocytozoon bieneusi an AIDS-related zoonotic pathogen John Hwa Lee College of Veterinary Medicine and Bio-Safety Research Institute Chonbuk National University Chonju 561-756 Korea A microsporidian parasite Enterocytozoon bieneusi is the most common microorganism recognized in AIDS patients and slow scientific progress is attributed to our inability to propagate the parasite. We report upon the development of a system of propagation using the pig biliary system. The parasite spores were continuously detected in the bile samples post onset of spore shedding in the gall bladder which suggests that this organism maintain persistent infection in the biliary system and that the hepatobiliary tree may represent a reservoir of infection. In conclusion the biliary tree is an adequate niche for the propagation of E. bieneusi. This work has also resulted in the development of a procedure of ultrasound-guided cholecystocentesis for aspirating biles. This is a simple and non-surgical procedure and creates no signs of clinical complications in the livers and the gall bladders after dozens of separate attempts. Thus this is a very useful and safe technique for the aspiration of bile from live animals. Key words Enterocytozoon bieneusi propagation immunosuppression biliary system cholecystocentesis. Introduction Microsporidia are obligate intracellular protozoan parasites that cause opportunistic infections in animals and humans especially in AIDS patients. They are sufficiently unique to be classified in a separate phylum. The phylum Microspora contain nearly 100 genera and more than 1000 species of microsporidia that infect a wide range of invertebrate and vertebrate hosts 4 . These organisms are defined by a nucleated sporoplasm a coiled polar tube an anchoring disk and the absence of several eukaryotic organelles such as mitochondria Golgi membranes and eukaryotic .

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