tailieunhanh - Báo cáo hóa học: " A Quick and Parallel Analytical Method Based on Quantum Dots Labeling for ToRCH-Related Antibodies"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: A Quick and Parallel Analytical Method Based on Quantum Dots Labeling for ToRCH-Related Antibodies | Nanoscale Res Lett 2009 4 1469-1474 DOI S11671-009-9422-7 NANO EXPRESS A Quick and Parallel Analytical Method Based on Quantum Dots Labeling for ToRCH-Related Antibodies Hao Yang Qing Guo Rong He Ding Li Xueqing Zhang Chenchen Bao Hengyao Hu Daxiang Cui Received 25 July 2009 Accepted 14 August 2009 Published online 3 September 2009 to the authors 2009 Abstract Quantum dot is a special kind of nanomaterial composed of periodic groups of II-VI III-V or IV-VI materials. Their high quantum yield broad absorption with narrow photoluminescence spectra and high resistance to photobleaching make them become a promising labeling substance in biological analysis. Here we report a quick and parallel analytical method based on quantum dots for ToRCH-related antibodies including Toxoplasma gondii Rubella virus Cytomegalovirus and Herpes simplex virus type 1 HSV1 and 2 HSV2 . Firstly we fabricated the microarrays with the five kinds of ToRCH-related antigens and used CdTe quantum dots to label secondary antibody and then analyzed 100 specimens of randomly selected clinical sera from obstetric outpatients. The currently prevalent enzyme-linked immunosorbent assay ELISA kits were considered as golden standard for comparison. The results show that the quantum dots labeling-based ToRCH microarrays have comparable sensitivity and specificity with ELISA. Besides the microarrays hold distinct advantages over ELISA test format in detection time cost operation and signal stability. Validated by the clinical assay our quantum dots-based ToRCH microarrays have H. Yang Q. Guo R. He X. Zhang C. Bao H. Hu D. Cui H Department of Bio-Nano Science and Engineering Key Laboratory for Thin Film and Microfabrication Technology of Ministry of Education National Key Laboratory of Micro Nano Fabrication Technology Research Institute of Micro Nano Science and Technology Shanghai Jiao Tong University 200240 Shanghai People s Republic of China e-mail dxcui@ D. Li Center of Biological .