tailieunhanh - Báo cáo hóa học: " Fabrication of Anti-human Cardiac Troponin I Immunogold Nanorods for Sensing Acute Myocardial Damage"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Fabrication of Anti-human Cardiac Troponin I Immunogold Nanorods for Sensing Acute Myocardial Damage | Nanoscale Res Lett 2009 4 1428-1433 DOI S11671-009-9415-6 NANO EXPRESS Fabrication of Anti-human Cardiac Troponin I Immunogold Nanorods for Sensing Acute Myocardial Damage Z. R. Guo C. R. Gu X. Fan Z. P. Bian H. F. Wu D. Yang N. Gu J. N. Zhang Received 1 July 2009 Accepted 9 August 2009 Published online 21 August 2009 to the authors 2009 Abstract A facile rapid solution-phase method of detecting human cardiac troponin I for sensing myocardial damage has been described using gold nanorods-based biosensors. The sensing is demonstrated by the distinct change of the longitudinal surface plasmon resonance wavelength of the gold nanorods to specific antibodyantigen binding events. For a higher sensitivity the aspect ratio of gold nanorods is increased up to ca by simply adding small amount of HCl in seed-mediated growth solution. Experimental results show that the detecting limit of the present method is 10 ng mL. Contrast tests reveal that these gold nanorods-based plasmonic biosensors hold much higher sensitivity than that of conventionally spherical gold nanoparticles. Keywords Gold nanorod Biosensor Surface modification Cardiac troponin I C. R. Gu is the co-first author. Electronic supplementary material The online version of this article doi s11671-009-9415-6 contains supplementary material which is available to authorized users. Z. R. Guo C. R. Gu Z. P. Bian H. F. Wu D. Yang J. N. Zhang El The Research Institute of Cardiovascular Disease The First Affiliated Hospital of Nanjing Medical University 210029 Nanjing China e-mail jinanzh506@ Z. R. Guo X. Fan N. Gu State Key Laboratory of Bioelectronics and Jiangsu Laboratory for Biomaterials and Devices Southeast University 210029 Nanjing China Introduction Cardiac troponin I cTnI is a protein subunit of cardiac troponin complex. During the myocardial damage process the troponin complex is broken up and the individual protein components including cTnI are released into the bloodstream 1 . .