tailieunhanh - Báo cáo hóa học: " In Situ Loading of Basic Fibroblast Growth Factor Within Porous Silica Nanoparticles for a Prolonged Release"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: In Situ Loading of Basic Fibroblast Growth Factor Within Porous Silica Nanoparticles for a Prolonged Release | Nanoscale Res Lett 2009 4 1297-1302 DOI S11671-009-9395-6 NANO EXPRESS In Situ Loading of Basic Fibroblast Growth Factor Within Porous Silica Nanoparticles for a Prolonged Release Jin Zhang Lynne-Marie Postovit Dashan Wang Richard B. Gardiner Richard Harris Mumin Md Abdul Anu Alice Thomas Received 12 March 2009 Accepted 8 July 2009 Published online 25 July 2009 to the authors 2009 Abstract Basic fibroblast growth factor bFGF a protein plays a key role in wound healing and blood vessel regeneration. However bFGF is easily degraded in biologic systems. Mesoporous silica nanoparticles MSNs with well-tailored porous structure have been used for hosting guest molecules for drug delivery. Here we report an in situ route to load bFGF in MSNs for a prolonged release. The average diameter d of bFGF-loaded MSNs is 57 8 nm produced by a water-in-oil microemulsion method. The in vitro releasing profile of bFGF from MSNs in phosphate buffer saline has been monitored for 20 days through a colorimetric enzyme linked immunosorbent assay. The loading efficiency of bFGF in MSNs is estimated at 3 . In addition the cytotoxicity test indicates that the MSNs are not toxic even at a concentration of 50 g mL. It is expected that the in situ loading method makes the MSNs a new delivery system to deliver protein drugs . growth factors to help blood vessel regeneration and potentiate greater angiogenesis. J. Zhang El M. M. Abdul A. A. Thomas Department of Chemical and Biochemical Engineering University of Western Ontario London ON N6A 5B9 Canada e-mail jzhang@ . Postovit Department of Anatomy and Cell Biology The Schulich School of Medicine and Dentistry University of Western Ontario London ON N6A 5C1 Canada D. Wang National Research Council Canada Institute for Chemical Process and Environmental Technology 1200 Montreal Road Ottawa ON K1A 0R6 Canada R. B. Gardiner R. Harris Department of Biology University of Western Ontario London ON N6A 5B7 Canada Keywords In