tailieunhanh - Báo cáo hóa học: " Micro/Nanoscale Parallel Patterning of Functional Biomolecules, Organic Fluorophores and Colloidal Nanocrystals"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Micro/Nanoscale Parallel Patterning of Functional Biomolecules, Organic Fluorophores and Colloidal Nanocrystals | Nanoscale Res Lett 2009 4 1222-1229 DOI s11671-009-9386-7 NANO EXPRESS Micro Nanoscale Parallel Patterning of Functional Biomolecules Organic Fluorophores and Colloidal Nanocrystals S. Sabella V. Brunetti G. Vecchio A. Della Torre R. Rinaldi R. Cingolani P. P. Pompa Received 8 May 2009 Accepted 1 July 2009 Published online 14 July 2009 to the authors 2009 Abstract We describe the design and optimization of a reliable strategy that combines self-assembly and lithographic techniques leading to very precise micro- nanopo-sitioning of biomolecules for the realization of micro- and nanoarrays of functional DNA and antibodies. Moreover based on the covalent immobilization of stable and versatile SAMs of programmable chemical reactivity this approach constitutes a general platform for the parallel site-specific deposition of a wide range of molecules such as organic fluorophores and water-soluble colloidal nanocrystals. Keywords Molecular self-assembly Lithographic techniques DNA Green fluorescent proteins Colloidal nanocrystals Abbreviations SAMs MPTS APTES NCs FM SiO2 Self-assembled monolayers Mercaptopropyltriethoxysilane Aminopropyltriethoxysilane Colloidal nanocrystals Fluorescein-5-maleimide Silicon dioxide Electronic supplementary material The online version of this article doi s11671-009-9386-7 contains supplementary material which is available to authorized users. S. Sabella R. Cingolani P. P. Pompa El Italian Institute of Technology IIT National Nanotechnology Laboratory Via Barsanti 1 Arnesano Lecce 73010 Italy e-mail V. Brunetti G. Vecchio A. D. Torre R. Rinaldi National Nanotechnology Laboratory of CNR-INFM Scuola Superiore ISUFI University of Salento Via Arnesano 16 Lecce 73100 Italy PMMA Polymethylmethacrylate GFP Green fluorescent protein GTA Glutaraldehyde Introduction Considerable efforts have recently been devoted by the nanoscience community to develop reliable patterning methodologies for the spatially controlled .

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